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Peptide chain elongation was achieved using a coupling solution consisting of preactivated amino acids (aa, 0.5 M) with ethyl 2-cyano-2 (hydroxyimino) acetate (oxyma, 1 M) and N,N-diisopropylcarbodiimide (DIC, 1 M) in DMF (1:1:1, aa:oxyma:DIC)

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As a key stress signal, ROS oxidizes the mitochondrial outer membrane protein Tom20, altering its structure and enhancing its ability to recruit Bax