Inflamm Bowel Dis (2024) 8:20814
10.1007/s11160-005-7846-4 42 MechlaouiM.DominguezD.RobainaL.GeraertP
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Each variable was scored for each sample slide, and we used a common scoring system where [0] = no alteration, [1] = mild changes, [2] = moderate changes, and [3] = severe changes ( 2.8 Immunohistochemical analysis Paraffin sections from different groups of liver tissues from rats were stained by immunohistochemistry (IHC) according to the manufacturing protocol using Anti-caspase 3 antibody (ab4051), at dilution 1:1,000, Anti-Bax antibody (ab32503), at dilution 1:250 and Anti-Bcl-2 antibody (ab182858), at dilution 1:500, (abcam, Cambridge, United Kingdom)