Walker explains that the approach was "pretty novel" and they made it sound safe in the grant application to secure funding, they understood the hormones and their mechanisms of action, but in reality, no one had done this kind of plant-based research before
For specific injuries like tennis elbow, this approach makes intuitive sense and aligns with how the peptide is most commonly used in research settings
[1] Acute kidney injury has been reported, particularly in patients experiencing severe gastrointestinal adverse reactions with volume depletion
For first-time users, start at 0.6mg per dose to monitor for side effects, and if appetite suppression is insufficient, gradually increase the dose each week by 0.6mg up to a maximum of 3.0mg
The resulting polypeptides were then eluted and precipitated with salt which provided a new, intermediate starting material that was then separated with chromatography (Jrnvall, Agerberth, Zasloff)