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Model and physiological RS 2 and RSSR substrates are, for example, bis(2-hydroxyethyl)disulfide (HEDS) 1,3,12,14,30 , l-cystine 1,3 , redox-sensitive yellow or green fluorescent protein 2 (rxYFP or roGFP2) 14,15,3135 , insulin 36,37 , collapsin response mediator protein 2 38,39 , the transcription factor OxyR 40 , 3-phosphoadenosine 5-phosphosulfate reductase (PR) 41,42 , methionine sulfoxide reductase (MSR) 34,43,44 , and ribonucleotide reductase (RNR) 2,34,4547

Total ascorbate concentrations were determined in a 1 ml mixture containing 200 l of TCA extract, 62.5 mM of potassium phosphate buffer (pH 7.4), 3.75 mM of EDTA, and 1.25 mM of dithiothreitol (DTT)

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10.3390/pathophysiology29010011 66