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Potency decreases over time even under optimal storage

103,104,105,106 However, a variety of stimuli, including nutrient perturbation, genotoxic stress, infection, pollutants and xenobiotics, trigger ROS overproduction, resulting in excessive oxidant challenge (oxidative distress)

2.12 Shotgun metagenomic sequencing Fecal DNA ( n = 3/group) was extracted using a FastPure Stool DNA Isolation Kit (Magnetic Beads, MJYH, China), and its quality was assessed for concentration, purity, and integrity prior to fragmentation (400 bp) using a Covaris M220

Since the exact risk factors may be hard to establish, treatment can be challenging [15]

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