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IGF-1 LR3 has been investigated in preclinical studies for its potential role in supporting tendon and ligament repair, as IGF-1 promotes cell proliferation, collagen synthesis, and tissue regeneration

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Colonic cells positive for INSL5, 5-hydroxytryptamine (5-HT, serotonin), GCG and GFP (for details of antibodies used, see Electronic supplementary material [ESM] Table 1) staining were manually counted and colocalisation assessed using a CellDiscoverer7 (Zeiss, Germany) and imaged using an SP8 confocal microscope (Leica Microsystems, Germany) with a 63 objective lens

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Later studies found similar patterns in Alzheimer's disease, Parkinson's disease, and COVID-19