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Besnard P, Passilly-Degrace P, Khan NA

After dispensing into 96-well white plates, a baseline read of luminescent signal was serially recorded over 5 min using a Flexstation 3 instrument (Molecular Devices) at 37C before addition of the indicated concentration of ligand, after which signal was repeatedly recorded for 30 min

Metabolic rate affects how quickly the body processes eliminated fat

Once the receptor opens to the G protein, it couples mainly to the stimulatory G protein Gs, which activates adenylyl cyclase and raises intracellular cyclic AMP

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